proteoextract subcellular proteome extraction kit (Millipore)
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proteoextract subcellular proteome extraction kit
Proteoextract Subcellular Proteome Extraction Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteoextract+subcellular+proteome+extraction+kit/proteoextract+subcellular+proteome+extraction+kit/us12365898-448-96-101
Average 90 stars, based on 1 article reviews
Proteoextract Subcellular Proteome Extraction Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteoextract+subcellular+proteome+extraction+kit/proteoextract+subcellular+proteome+extraction+kit/us12365898-448-96-101
Average 90 stars, based on 1 article reviews
proteoextract subcellular proteome extraction kit - by Bioz Stars,
2026-09
90/100 stars
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Extraction:Article Title: Phosphorylation at the disordered N-end makes HuR accumulate and dimerize in the cytoplasm. Article Snippet: .. Separation of 3 × 10 6 HeLa cell extracts into cytosol and nucleus fractions was performed with a Article Title: Phosphorylation at the disordered N-end makes HuR accumulate and dimerize in the cytoplasm Article Snippet: .. Separation of 3 × 10 6 HeLa cell extracts into cytosol and nucleus fractions was performed with a Article Title: Wnt/β-catenin activation by mutually exclusive FBXW11 and CTNNB1 hotspot mutations drives salivary basal cell adenoma. Article Snippet: .. Article Title: Calcium channel 3.2 inhibitory peptides and uses thereof Article Snippet: .. Immunoblot Cell lysates from cultivated cells lines and DRG tissue lysates from pooled L4/L5 DRG were prepared using 1× RIPA ice-cold buffer (20 mm Tris-HCl pH 7.4, 150 mm NaCl, 1% Nonidet P-40, 1% sodium deoxycholate, 0.1% SDS, with 0.1% Triton X100 and protease inhibitor cocktail) and rotated at 4° C. for 1 h before the supernatant was extracted by centrifugation at 12,000 g at 4° C. for 5 min. To examine the subcellular localization of Cav3.2, NG108-15 cells and DRG tissue were homogenized and then fractionated to obtain plasma membrane, cytosolic, and nuclear using the Article Title: <scp>CD59</scp> double knockout mice express a <scp>CD59ba</scp> hybrid fusion protein that mediates insulin secretion Article Snippet: .. Subcellular fractions were retrieved using the Article Title: MDM2 inhibitors induce apoptosis by suppressing MDM2 and enhancing p53, Bax, Puma and Noxa expression levels in imatinib‑resistant chronic myeloid leukemia cells Article Snippet: The protein concentrations in the lysates were measured utilizing a BCA protein assay kit (cat. no. T9300A; Takara Bio, Inc.). .. The extraction of cells was carried out utilizing the Article Title: MDM2 inhibitors induce apoptosis by suppressing MDM2 and enhancing p53, Bax, Puma and Noxa expression levels in imatinib‑resistant chronic myeloid leukemia cells Article Snippet: The extraction of cells was carried out utilizing the ProteoExtract Subcellular Proteome Extraction Kit from MilliporeSigma (cat. no. 539790), following the manufacturer's guidelines, and the analysis was performed through western blotting, as previously reported ( ). .. Briefly, using the Article Title: Overexpression of miR-29a and miR-29b is involved in imatinib resistance via abrogated NF1 expression and increased ERK1/2 activation in chronic myeloid leukemia cells. Article Snippet: Breakpoint cluster region::Abelson 1 (BCR::ABL1) tyrosine kinase inhibitors (TKIs), such as imatinib, are used to treat chronic myeloid leukemia (CML), but BCR::ABL1 TKI resistance develops in 20–30% of affected patients, which poses a serious clinical problem.. MicroRNAs (miRNAs) have been related to the development and aggravation of CML and BCR::ABL1 TKI resistance; however, the underlying mechanisms remain unknown.. In this study, we explored the roles of miRNAs in imatinib resistance as well as the underlying mechanism in imatinib-resistant K562 (K562/IR) cells. Western Blot:Article Title: Calcium channel 3.2 inhibitory peptides and uses thereof Article Snippet: .. Immunoblot Cell lysates from cultivated cells lines and DRG tissue lysates from pooled L4/L5 DRG were prepared using 1× RIPA ice-cold buffer (20 mm Tris-HCl pH 7.4, 150 mm NaCl, 1% Nonidet P-40, 1% sodium deoxycholate, 0.1% SDS, with 0.1% Triton X100 and protease inhibitor cocktail) and rotated at 4° C. for 1 h before the supernatant was extracted by centrifugation at 12,000 g at 4° C. for 5 min. To examine the subcellular localization of Cav3.2, NG108-15 cells and DRG tissue were homogenized and then fractionated to obtain plasma membrane, cytosolic, and nuclear using the Article Title: MDM2 inhibitors induce apoptosis by suppressing MDM2 and enhancing p53, Bax, Puma and Noxa expression levels in imatinib‑resistant chronic myeloid leukemia cells Article Snippet: The protein concentrations in the lysates were measured utilizing a BCA protein assay kit (cat. no. T9300A; Takara Bio, Inc.). .. The extraction of cells was carried out utilizing the Protease Inhibitor:Article Title: Calcium channel 3.2 inhibitory peptides and uses thereof Article Snippet: .. Immunoblot Cell lysates from cultivated cells lines and DRG tissue lysates from pooled L4/L5 DRG were prepared using 1× RIPA ice-cold buffer (20 mm Tris-HCl pH 7.4, 150 mm NaCl, 1% Nonidet P-40, 1% sodium deoxycholate, 0.1% SDS, with 0.1% Triton X100 and protease inhibitor cocktail) and rotated at 4° C. for 1 h before the supernatant was extracted by centrifugation at 12,000 g at 4° C. for 5 min. To examine the subcellular localization of Cav3.2, NG108-15 cells and DRG tissue were homogenized and then fractionated to obtain plasma membrane, cytosolic, and nuclear using the Centrifugation:Article Title: Calcium channel 3.2 inhibitory peptides and uses thereof Article Snippet: .. Immunoblot Cell lysates from cultivated cells lines and DRG tissue lysates from pooled L4/L5 DRG were prepared using 1× RIPA ice-cold buffer (20 mm Tris-HCl pH 7.4, 150 mm NaCl, 1% Nonidet P-40, 1% sodium deoxycholate, 0.1% SDS, with 0.1% Triton X100 and protease inhibitor cocktail) and rotated at 4° C. for 1 h before the supernatant was extracted by centrifugation at 12,000 g at 4° C. for 5 min. To examine the subcellular localization of Cav3.2, NG108-15 cells and DRG tissue were homogenized and then fractionated to obtain plasma membrane, cytosolic, and nuclear using the Clinical Proteomics:Article Title: Calcium channel 3.2 inhibitory peptides and uses thereof Article Snippet: .. Immunoblot Cell lysates from cultivated cells lines and DRG tissue lysates from pooled L4/L5 DRG were prepared using 1× RIPA ice-cold buffer (20 mm Tris-HCl pH 7.4, 150 mm NaCl, 1% Nonidet P-40, 1% sodium deoxycholate, 0.1% SDS, with 0.1% Triton X100 and protease inhibitor cocktail) and rotated at 4° C. for 1 h before the supernatant was extracted by centrifugation at 12,000 g at 4° C. for 5 min. To examine the subcellular localization of Cav3.2, NG108-15 cells and DRG tissue were homogenized and then fractionated to obtain plasma membrane, cytosolic, and nuclear using the Membrane:Article Title: Calcium channel 3.2 inhibitory peptides and uses thereof Article Snippet: .. Immunoblot Cell lysates from cultivated cells lines and DRG tissue lysates from pooled L4/L5 DRG were prepared using 1× RIPA ice-cold buffer (20 mm Tris-HCl pH 7.4, 150 mm NaCl, 1% Nonidet P-40, 1% sodium deoxycholate, 0.1% SDS, with 0.1% Triton X100 and protease inhibitor cocktail) and rotated at 4° C. for 1 h before the supernatant was extracted by centrifugation at 12,000 g at 4° C. for 5 min. To examine the subcellular localization of Cav3.2, NG108-15 cells and DRG tissue were homogenized and then fractionated to obtain plasma membrane, cytosolic, and nuclear using the Electrophoresis:Article Title: MDM2 inhibitors induce apoptosis by suppressing MDM2 and enhancing p53, Bax, Puma and Noxa expression levels in imatinib‑resistant chronic myeloid leukemia cells Article Snippet: The extraction of cells was carried out utilizing the ProteoExtract Subcellular Proteome Extraction Kit from MilliporeSigma (cat. no. 539790), following the manufacturer's guidelines, and the analysis was performed through western blotting, as previously reported ( ). .. Briefly, using the |